Bước đầu ứng dụng công nghệ enzyme để trích ly các hoạt chất thứ cấp từ rễ cây đảng sâm (codonopsic javanica)

Abstract

Đảng sâm Codonopsis javanica (Blume) Hook.f. mainly distributed in the highland mountain region in Viet Nam, concentrated mostly in Kontum province. In this study, for the first time Viscozyme (Novozyme) was used to extract and determine the content of saponin triterpenoid from its root. Saponin triterpenoid is one of the medicinal components concentrated mainly in tuberous roots. The results showed that when using Viscozyme extraction at 300C for 1 hour, the enzyme concentration was 0.4%, the ratio of material: enzyme was 1:7 (g:ml), the amount of triterpenoid saponin obtained was 88.7 ± 5.2 mg / 100g 1.5 times higher not using the enzyme under the same conditions.